This book constitutes the proceedings of the 16th Asian Conference on Intelligent Information and Database Systems, ACIIDS 2024, held in Ras Al Khaimah, UAE, during April 15–18, 2024. The 58 full papers are presented in this book were carefully reviewed and selected from 251 submissions. They are organized in topical sections as follows: Part One: AI-driven Cybersecurity Solutions; AI-driven Medical Analytics; Computational Intelligence; and Data Modelling and Information Systems. Part Two: Image and Video Processing; Prediction and Recommendation Systems; and Text, Speech and Natural Language Processing.