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Scientific Essay from the year 2016 in the subject Biology - Micro- and Molecular Biology, , language: English, abstract: The colonial diameter of Lactococcus lactis spp. lactis isolates on MRS agar was promoted by providing negative atmospheric pressure and /or enrichment of negative atmosphere by CO2 gas in vacuum desiccator. In this study, application of negative atmospheric pressure or enrichment of negative atmosphere by CO2 gas at 722 mmHg was observed for maximum (2.5 mm) colony size of L. lactis spp. lactis on agar surface. No significant difference was observed in the colonial diameter in the presence of negative atmospheric pressure or enrichment of negative atmosphere by CO2 gas at 722 to 608 mmHg pressure.