This publication explores the multifaceted applications of Methylene Blue (MB) in modern oncology, focusing on its role in advanced imaging and therapeutic strategies. The book details MB-mediated in vivo fluorescence and photoacoustic imaging, alongside light-induced cancer therapy, systematically describing its optical properties and photothermal effects. It highlights MB as a biocompatible and environmentally friendly material with a century-long history of clinical and biomedical use, extending its utility to diverse fields beyond medicine.

This work is positioned as a landmark publication due to its comprehensive coverage of Methylene Blue's advanced applications in cancer imaging and therapy. The detailed exploration of MB-mediated fluorescence, photoacoustic imaging, and light-induced therapy, alongside its optical and photothermal properties, suggests a significant contribution to the field. The book's focus on a material with a long history of clinical use, while highlighting novel applications, indicates its potential value to researchers and clinicians. Its inclusion of MB's broader industrial applications further contextualizes its versatility.