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The First Of Two New Volumes Covering Mitochondria, This Volume Presents Modern Methods That Have Been Developed To Examine Mitochondrial Electron Transport Chain Complexes, Iron-sulfur Proteins And Reactive Oxygen Species. These New Techniques Provide Investigators With Sensitive, Original Approaches To The Study Of Disease States Associated With Mitochondrial Malfunction. The Critically Acclaimed Laboratory Standard For 40 Years, Methods In Enzymology Is One Of The Most Highly Respected Publications In The Field Of Biochemistry. Since 1955, Each Volume Has Been Eagerly Awaited, Frequ Front Cover; Methods In Enzymology: Mitochondrial Function, Part A: Mitochondrial Electron Transport Complexes And Reactive Oxygen Species; Copyright Page; Contents; Contributors; Preface; Methods In Enzymology; Chapter 1: Visualizing Functional Flexibility By Three-dimensional Electron Microscopy: Reconstructing Complex I Of The Mitochondrial Respiratory Chain; 1. Introduction; 2. Random Conical Reconstruction; 3. Application Of The Techniques To Complex I From Yarrowia Lipolytica; 4. Conclusion; Acknowledgments; References Chapter 2: Correlated Light And Electron Microscopy/electron Tomography Of Mitochondria In Situ1. Introduction; 2. Light Microscopy; 3. Electron Microscope Tomography; Acknowledgments; References; Chapter 3: Studies Of Complex I By Fourier Transform Infrared Spectroscopy; 1. Spectroscopic Studies Of Complex I; 2. Ftir Difference Spectroscopy And Its Application To Complex I; 3. Preparation Of Rehydrated Layers Of Complex I For Atr-ftir Spectroscopy; 4. Analysis Of Rehydrated Complex I Layers By Perfusion-induced Atr-ftir Difference Spectroscopy 5. Analysis Of Rehydrated Complex I Layers By Electrochemically-induced Atr-ftir Difference Spectroscopy6. Model Compounds And Strategies For Interpretation; 7. Example Ir Difference Spectra Of Complex I; Acknowledgments; References; Chapter 4: Electron Transfer In Respiratory Complexes Resolved By An Ultra-fast Freeze-quen