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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1895 edition. Excerpt:...bacilli first, and that the phagocytes become active later. Eventually take off the cover-glass, carefully allow the film to dry, and stain it with eosine and methylene-blue in the usual manner. Examine it with-in-oil immersion, and note especially the relation of the eosinophile cells and phagocytes to the bacilli. Chemiotaxis (a) Inject a small quantity of an old broth culture of Bacillus pyocyaneus under the skin of the thigh of a pithed frog. (6) Four hours later remove some of the lymph and prepare films. (e) Stain these in the usual manner with eosine and methylene-blue. Examine with a high power and-in. oil immersion. Note the large number of eosinophile cells attracted (" positive chemiotaxis "). PAET II BACTERIOLOGICAL ANALYSIS LESSONS I-XII LESSONS I And II Cleaning and Sterilisation of Tubes and Flasks--Preparation of Nutrient Media--Beef Broth--Glycerine Broth--Grape-Sugar Broth--Meat Infusion--Gelatine--Grape-Sugar Gelatine--25 per cent Gelatine--Carbolic Acid Gelatine--Peptone Solution--Potato Tubes--Milk Tubes--Agar-Agar--Glycerine Agar-Agar--Grape-Sugar Agar-Agar--Serum Tubes. Cleaning and Sterilisation of Test-Tubes, Flasks, and Beakers I. Cleaning Of New Test-tubes. (1) Roll a little cotton-wool round a strong glass rod and fasten it with a piece of strong thread or string. (2) Dip the cotton-wool in strong nitric acid, and thoroughly rub the inside of each tube. (3) Wash the tubes three or four times in water, to remove all trace of the acid. (4) Allow them to drain, until they are nearly dry. (5) Rinse each tube with a little absolute alcohol or strong spirit. (6) Again allow the tubes to drain. (7) When quite dry, they are ready for sterilisation. G II. Cleaning Of Test-tubes Which Have Already BEEN USED....