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CONTENTS: Preamble, Trace minerals, Vitamins - Thiamine (Vitamin B1), Ribloflavin (Vitamin B2), Nicotinic acid (Niacin), Pantothenic acid (Coenzyme A), Pyridoxine (Vitamin B6), Biotin, Folic acid, Cyanocobalamin (Vitamin B12), Ascorbic acid (Vitamin C), Inositol, Choline, Retinoic acid (Vitamin A), Vitamin D (Calciferol), Vitamin E (Tocopherol), Vitamin K, Vitamin requirements for fish, Dietary sources of vitamins, Present status of research on Indian major carps, Estimation of micronutrients, Estimation of Vitamin-C in feed, Principle for the estimation of Riboflavin in feed, Principle for the estimation of folic acid in feed, Principle for the estimation of Thiamine in feed, Estimation of trace minerals in feed, Determination of Fe, Mn, Zn and Cu by AAS in fish feed, References.