This book constitutes the refereed proceedings of the 7th International Conference on Pattern Recognition in Bioinformatics, PRIB 2012, held in Tokyo, Japan, in November 2012. The 24 revised full papers presented were carefully reviewed and selected from 33 submissions. Their topics are widely ranging from fundamental techniques, sequence analysis to biological network analysis. The papers are organized in topical sections on generic methods, visualization, image analysis, and platforms, applications of pattern recognition techniques, protein structure and docking, complex data analysis, and sequence analysis.