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The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatoryenzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here werechosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, andcell physiology.
This manual investigates the practical application of enzyme histochemistry as a primary tool for the functional analysis of macromolecular structures within cells and tissues. The authors, Cornelis J. Van Noorden and Wilma M. Fredericks, leverage their expertise in microscopy to curate a collection of reliable, high-precision techniques. The text provides both the theoretical framework necessary for understanding enzymatic activity and the procedural rigor required for modern laboratory research.
What You Will Find
Experts recognize this volume as a standard reference for researchers seeking to implement histochemical methods in a laboratory setting. Readers frequently note the clarity of the protocols and the utility of the introductory theoretical chapters for those new to the field.
Page Count:
128
Publication Date:
1992-01-01
Publisher:
Oxford Univ Pr
ISBN-10:
0198564341
ISBN-13:
9780198564348
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