This book provides a comprehensive guide to the techniques and methodologies essential for understanding protein expression in mammalian cells. It addresses the growing importance of protein research in advancing cell manipulation and disease intervention, building upon recent progress in high-throughput DNA sequencing. The text is structured to offer clear introductions to key topics, detailed lists of materials and reagents, and step-by-step protocols designed for reproducibility. It also includes troubleshooting advice to help researchers navigate potential challenges in their work.

This book is presented as a compendium of vital techniques from expert researchers in the field of mammalian protein expression. Its structure, following the established "Methods in Molecular Biology" format, suggests a focus on practical, reproducible laboratory protocols. The content is intended to aid scientists in deepening their understanding of cellular biology through the study of proteins.