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Excerpt from Laboratory Notes of Technical Methods for the Nervous System If specimens are not thoroughly hardened in Muller's uid, the subsequent permanent preservation in strong alco hol does them considerable injury. The alcohol slowly dis solves out the chrome salts and changes the myelin. In the course of a year or two cholesterin crystals form in the alco hol, and minute cavities and vacuoles appear in the speci mens and they do not stain well. If for any reason it is necessary to examine specimens before they are thoroughly hardened, it is best to keep the material for permanent preservation on the shelves in water containing camphor, or dilute, thirty to forty per cent., solutions of alcohol. In the laboratory even thoroughly hardened material is not kept permanently in strong alcohol. Seventy or eighty per cent. About the Publisher Forgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.com This book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully; any imperfections that remain are intentionally left to preserve the state of such historical works.