This volume presents the proceedings of the 16th International Conference on Software Business (ICSOB 2025), focusing on the theme 'Software Business in Digital Societies'. The conference, held in Stuttgart, Germany, from November 24-26, 2025, gathered researchers and practitioners to discuss the evolving landscape of software business. The proceedings include 18 full papers and 11 short papers, carefully selected from 65 submissions, covering a wide array of topics crucial to understanding and advancing software business in contemporary digital environments.

This collection of papers offers a comprehensive overview of current research and practice in software business, particularly within the context of digital societies. The diverse topical sections indicate a broad scope, addressing critical areas such as data economy, AI adoption, sustainability, and ethical considerations. The selection process, involving a review of 65 submissions, suggests a high standard for the included contributions, making this volume a valuable resource for academics and industry professionals interested in the future of software business.