
The colloidal gold probe is the most recent immunocytochemical marking technique to have been developed for the detection of antigens in situ. The major advantages of colloidal gold probes are that they are small, dense probes of wide applicability in the biological sciences. The probe canbe used for the localization of specific proteins in conjunction with a wide range of microscopical and non-microscopical techniques. This book aims to describe the established techniques of gold probe production and immunolabelling. Each technique is summarized in a table which can be used in thelaboratory without recourse to the text. Original references are also given to enable readers to trace the origins of each technique. The book can therefore be used both as a bench-top manual and as a guide to further reading.
This text investigates the utility and application of colloidal gold probes as a primary method for immunocytochemical marking in biological research. Author Julian E. Beesley provides a comprehensive overview of the production and implementation of these probes, drawing upon established laboratory protocols to assist researchers in the precise localization of antigens. The book serves as both a technical manual and a reference guide, organizing complex methodologies into accessible formats for practical use in the laboratory.
What You Will Find
Experts and laboratory practitioners recognize this handbook as a foundational resource for those utilizing immunocytochemical marking techniques. Readers frequently note the practical utility of the tabular summaries, which allow for efficient application of complex procedures in a busy laboratory environment.
Page Count:
64
Publication Date:
1989-01-01
Publisher:
Oxford University Press, USA
ISBN-10:
019856418X
ISBN-13:
9780198564188
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