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Our understanding of the functions of epithelia, and their development and maintenance, has been rapidly advanced by the use of cell cultures which faithfully display the morphological, biochemical, and functional characteristics of these tissues. This book gives detailed step-by-step descriptions of the methods used to mimic epithelial and endothelial tissues in vitro, and the techniques used to characterize these cell culture models, together with hints and tips for success, troubleshooting guides, and key background information. Epithelial Cell Culture: A Practical Approach is a timely and comprehensive practical guide for all researchers who are studying, or intend to study, epithelia-related tissues using modern cell culture techniques. As such, this book will have a very wide appeal to cell biologists, developmental biologists, biochemists, pharmaceutical scientists, pharmacologists, and toxicologists.
This book investigates the methodological requirements for establishing and maintaining in vitro epithelial and endothelial cell models that accurately reflect the physiological characteristics of living tissues. Andrew J. Shaw provides a comprehensive framework for researchers, synthesizing established laboratory protocols with practical troubleshooting strategies. The text serves as a technical manual designed to bridge the gap between theoretical understanding of epithelial function and the practical application of cell culture techniques in a laboratory setting.
What You Will Find
Experts and researchers in the field of cell biology frequently cite this work as a reliable reference for standardized laboratory procedures. The text is noted for its technical clarity and its utility as a practical resource for both novice and experienced scientists working with tissue models.
Page Count:
218
Publication Date:
1996-07-15
Publisher:
IRL Press
ISBN-10:
0199635722
ISBN-13:
9780199635726
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