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This book provides a concise yet comprehensive account of animal cell cultures: how they are used in cell growth assays, how to interpret results, and how to avoid potential problem areas when using these commonly employed research tools. Cell Growth and Division furnishes laboratory protocols for using a wide range of animal cell lines. The book gives readers full coverage on measuring parameters of growth, mouse embryo cells, C3H 10T1/2 and NIHT3T cell lines, human leukemia cells, and normal human epidermal keratinocytes and mesothelial cells. This complete guide also offers convenient, all-in-one coverage of cloned T cell proliferation, muscle cell cultures, human diploid fibroblasts, cell synchronization, and BAL B/c3T3 cells. Laboratory investigators familiar with only one cell line will particularly appreciate the depth of coverage, which will make it possible to choose the line best suited to a given investigation. guidance on how they are carried out, how to interpret results, and how to avoid potential problem areas when using these commonly employed research tools.
This book investigates the practical methodologies required for the effective cultivation and analysis of animal cell lines in a laboratory setting. Renato Baserga, a recognized authority in cell biology, compiles a comprehensive framework for researchers to navigate the complexities of cell growth assays. The text serves as a technical manual, providing standardized protocols to ensure experimental accuracy and the mitigation of common procedural errors.
What You Will Find
Experts and laboratory investigators frequently cite this work as a foundational reference for those working with diverse animal cell cultures. The text is noted for its technical density and its utility as a practical, all-in-one resource for researchers seeking to standardize their experimental procedures.
Page Count:
172
Publication Date:
1989-06-08
Publisher:
Oxford University Press
ISBN-10:
0199630275
ISBN-13:
9780199630271
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