Molecular Genetics of Yeast: A Practical Approach
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Author Description
The yeast Saccharomyces cerevisiae is used extensively in academic research as a model eukaryote, and is also one of the most important industrial organisms. It is easy to manipulate, and has a great many parallels with higher organisms, making it ideal for studies in molecular biology, genetics, and biotechnology. This book describes current experimental procedures, written by internationally recognized experts, for working with this organism. Topics covered include DNA isolation, cloning and expression vectors, construction and use of DNA libraries, Ty insertional mutagenesis, high-efficiency transformation, cell-free translation of mRNAs, virus-like particles, and aspects of industrial strains.Molecular Genetics of Yeast: A Practical Approach provides a comprehensive compendium of detailed, clearly presented protocols, with additional practical tips, for all researchers working with this organism.
Menrva's Summary
THE THESIS
This volume addresses the fundamental question of how to effectively utilize Saccharomyces cerevisiae as a model organism for advanced molecular and genetic research. Edited by John R. Johnston, the text compiles contributions from internationally recognized experts to provide a standardized framework for experimental procedures. It serves as a technical manual, bridging the gap between theoretical genetic principles and the practical application of laboratory protocols in both academic and industrial settings.
THE SCOPE MAP
What You Will Find
- Detailed protocols for DNA isolation, cloning, and the construction of DNA libraries.
- Methodologies for high-efficiency transformation and Ty insertional mutagenesis.
- Technical guidance on cell-free translation of mRNAs and the analysis of virus-like particles.
- Practical considerations for the manipulation and study of industrial yeast strains.
THE AUTHORITY PERSPECTIVE
Researchers frequently cite this volume as a foundational resource for laboratory protocols involving yeast genetics. Experts highlight the clarity of the presented procedures, noting that it remains a standard reference for those engaged in eukaryotic molecular biology research.
Page Count:
312
Publication Date:
1994-08-04
Publisher:
Oxford University Press
ISBN-10:
0199634300
ISBN-13:
9780199634309
Science
Laboratory manuals
Genetics
Microbiology
Yeast
Biotechnology
