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Measuring metabolic rates is central to important questions in many areas of scientific research. Unfortunately these measurements are anything but straightforward, and numerous pitfalls await the novice and even the experienced investigator. Measuring Metabolic Rates de-mystifies the field, explaining every common variation of metabolic rate measurement, from century-old manometric methods through ingenious syringe-based techniques, direct calorimetry, aquatic respirometry, stable-isotope metabolic measurement and every type of flow-through respirometry. Each variation is described in enough detail to allow it to be applied in practice. Background information on different analyzer and equipment types allows users to choose the best instruments for their application. Respirometry equations - normally a topic of terror and confusion to researchers - are derived and described in enough detail to make their selection and use effortless. Vital topics such as manual and automated baselining, implementing multi-animal systems, and the correct analysis and presentation of metabolic data are covered in enough detail to turn a respirometry neophyte into a hardened metabolic warrior, ready to take on the task of publication in peer-reviewed journals.
This manual addresses the technical complexities and common pitfalls inherent in the accurate measurement of metabolic rates across diverse scientific disciplines. John R. B. Lighton provides a comprehensive framework for researchers, drawing on his extensive experience to demystify various respirometry techniques. The text serves as a bridge between theoretical physiological concepts and the practical application of instrumentation in laboratory and field settings. By standardizing the derivation and application of respirometry equations, the author aims to improve the quality and reproducibility of metabolic data in peer-reviewed research.
What You Will Find
Experts and researchers frequently cite this work as a foundational resource for anyone conducting metabolic studies. The text is noted for its ability to translate dense mathematical and technical requirements into accessible, actionable protocols for both novices and experienced investigators.
Page Count:
216
Publication Date:
2008-01-01
Publisher:
Oxford University Press
ISBN-10:
0199885931
ISBN-13:
9780199885930
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