This monograph investigates the complex processes and applications of protoplast fusion as a technique for genetic engineering in higher plants. Gleba, a recognized researcher in plant cell biology, draws upon extensive experimental data and theoretical frameworks to present a comprehensive overview of the field. The book details the methodologies for isolating plant protoplasts, inducing fusion, and regenerating viable hybrid plants, emphasizing the potential for creating novel plant varieties with desirable traits.

This work is recognized as a foundational text in the field of plant genetic engineering, particularly concerning somatic hybridization. Its detailed technical information and theoretical discussions make it an invaluable resource for researchers and advanced students in plant science and biotechnology. The monograph's comprehensive scope and rigorous scientific approach have cemented its status as a key reference for understanding the principles and practices of protoplast fusion.