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Free radicals, molecules with unpaired electrons, are highly reactive and play key roles in physiologic regulation and in many degenerative and pathologic processes, making them a fertile area of research. This book focuses on spin trapping, a sophisticated technique for the identification of free radicals in biological systems. The method is complex, and this book offers an in-depth guide to all of the critical aspects needed for its application to free radicals in biology. This includes advice on interpreting results, trouble-shooting, and experimental designs. The book looks at future directions in the field and will prove an invaluable resource for investigators working in the biology of free radicals, regardless of whether they are new or highly experienced in the applications of spin trapping.
This book investigates the technical application of spin trapping as a primary methodology for the identification and analysis of free radicals within biological systems. The authors, a team of established researchers in the field, synthesize current experimental protocols to address the inherent complexities of detecting highly reactive molecules. They provide a structured framework for researchers to navigate the challenges of experimental design, data interpretation, and procedural troubleshooting in laboratory settings.
What You Will Find
Experts in the field recognize this text as a technical manual for both novice and experienced investigators working with free radical biology. Readers frequently note the practical utility of the troubleshooting sections for refining complex experimental procedures.
Page Count:
464
Publication Date:
1999-10-14
Publisher:
Oxford University Press
ISBN-10:
0195095057
ISBN-13:
9780195095050
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