This volume investigates the technical methodologies required for the successful amplification of DNA and RNA within cellular and tissue environments. Edited by C. Simon Herrington and John J. O'Leary, the text compiles contributions from leading experts to provide a standardized framework for in situ molecular analysis. It addresses the inherent difficulties of maintaining morphological integrity while performing nucleic acid amplification, offering a comprehensive guide for laboratory practitioners.

Experts and laboratory professionals recognize this text as a foundational resource for molecular biologists and pathologists performing in situ analyses. Readers frequently note the technical density of the protocols, which are designed specifically for experienced researchers requiring precise, reproducible results in a clinical or academic setting.