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This volume provides detailed laboratory protocols, advice, hints and tips, example data, key literature citations, background information, and troubleshooting comments for the steps used in most molecular biology laboratories for example transformation, construction of cDNA and genomic libraries, probe preparation, analysis of gene organization and expression, in vitro mutagenesis, and DNA sequencing. The information provided is an completely up-to-date account of each topic by established researchers.These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.
This volume investigates the fundamental laboratory protocols and methodologies required to perform DNA cloning and molecular analysis in a modern research setting. Edited by B. D. Hames and D. M. Glover, the text compiles contributions from established researchers to provide a standardized framework for molecular biology techniques. It serves as a technical reference, integrating theoretical background with practical troubleshooting to ensure reproducibility in experimental outcomes.
What You Will Find
Experts recognize this text as a foundational resource for laboratory practitioners due to its comprehensive coverage of core molecular techniques. Readers frequently note the technical density of the prose, which is designed specifically for active researchers and students in the field of genetics.
Page Count:
288
Publication Date:
1995-06-15
Publisher:
IRL Press
ISBN-10:
0199634769
ISBN-13:
9780199634767
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