This book constitutes the refereed proceedings of the Second International Conference on Distributed Artificial Intelligence, DAI 2020, held in Nanjing, China, in October 2020. The 9 full papers presented in this book were carefully reviewed and selected from 22 submissions. DAI aims at bringing together international researchers and practitioners in related areas including general AI, multiagent systems, distributed learning, computational game theory, etc., to provide a single, high-profile, internationally renowned forum for research in the theory and practice of distributed AI. Due to the Corona pandemic this event was held virtually.