Single-Molecule Studies of Nucleic Acids and Their Proteins
0
0
Author Description
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
Menrva's Summary
THE THESIS
This text investigates the physical properties of nucleic acids and the experimental methodologies required to observe their interactions with proteins at the single-molecule level. The authors, a team of researchers specializing in biophysics and molecular mechanics, synthesize current knowledge on DNA and RNA elasticity with the technical application of advanced trapping and imaging technologies. By focusing on specific enzyme families, the work establishes a framework for applying these biophysical tools to complex biological systems.
THE SCOPE MAP
What You Will Find
- Elastic properties of DNA and RNA molecules
- Technical operation of optical, magnetic, and acoustic tweezers
- Principles of fluorescence microscopy, FCS, and FRET
- Case studies involving polymerases, helicases, and topoisomerases
Scope Limits
- The text does not provide an exhaustive survey of all known single-molecule techniques
- It does not cover broader cellular-level biological processes outside of specific protein-nucleic acid interactions
THE AUTHORITY PERSPECTIVE
Experts identify this text as a specialized resource for graduate-level students and researchers entering the field of single-molecule biophysics. Readers frequently note that the prose maintains a high level of technical rigor while successfully bridging the gap between theoretical physics and practical laboratory application.
Page Count:
240
Publication Date:
2018-01-01
Publisher:
OUP Oxford
ISBN-10:
0192559966
ISBN-13:
9780192559968
Science
Nucleic Acids
Genetics
Biochemistry
Earth Sciences
Academic
Nuclear Physics
Biophysics