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Product Description Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, forpreparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches tocombining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control givevalid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples. Review "This book is very useful not only for immunologists but also for amny specialists in chemistry and biology and is certainly recommendable" Immunological Investigations About the Author Jim Gosling, Department of Biochemistry, National University of Ireland.
This volume addresses the fundamental challenge of developing and optimizing reliable immunoassays for a wide range of analytes in biological research. Author James P. Gosling, a specialist in biochemistry at the National University of Ireland, provides a comprehensive technical framework for researchers. The text integrates theoretical principles with practical laboratory procedures, offering a systematic guide to antibody characterization, reagent preparation, and assay validation. By bridging the gap between commercial availability and custom development, the book serves as a technical manual for scientists across various biological and chemical disciplines.
What You Will Find
Experts and reviewers in the field of immunology recognize this work as a highly practical resource for specialists in chemistry and biology. Readers frequently note the clarity of the technical instructions and the utility of the provided quality assurance methods for maintaining valid experimental results.
Page Count:
304
Publication Date:
2000-01-01
Publisher:
Oxford University Press
ISBN-10:
0199637113
ISBN-13:
9780199637119
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