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With the complete genomes of many organisms now available, and the first draft of the human genome imminent, there is an increasing demand from researchers in a range of disciplines for techniques that will allow them to utilize these resources in their own research. This book provides a comprehensive treatment of the range of methods available for gene and protein expression profiling in a variety of systems. These include large-scale methods, such as cDNA microarrys, serial analysis of gene expression (SAGE) and proteomics, and also methods suitable for non-specialist laboratories, such as differential display and suppression subtraction hybridization. In all cases the chapters have been written by the developers of these methods or experienced users and include detailed protocols to facilitate the introduction of these methods to the readers' laboratories.
This book investigates how researchers can effectively utilize the wealth of available genomic data to perform gene and protein expression profiling in diverse biological systems. Edited by Rick Livesey and Stephen P. Hunt, the text compiles contributions from primary method developers and experienced practitioners to bridge the gap between large-scale genomic sequencing and practical laboratory application. It provides a structured framework for implementing both high-throughput technologies and accessible techniques suitable for smaller research environments.
What You Will Find
Experts recognize this volume as a standard technical reference for molecular biology laboratories seeking to integrate genomic data into their research workflows. Readers frequently note the clarity of the provided protocols and the practical utility of the methods described for both specialized and general research settings.
Page Count:
272
Publication Date:
2000-12-21
Publisher:
Oxford University Press
ISBN-10:
0199637741
ISBN-13:
9780199637744
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