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Polypeptide hormones play a central role in the regulation of biological processes from the minute-to-minute control of vital functions to the programming of birth, development, reproduction, and ageing. The first of a two-volume work on the methods of study used in this field, Peptide Hormone Secretion focuses on the isolation of polypeptide hormones and their identification and structural analysis, the development of sensitive bioassay and immunoassay techniques, and tissue localization of peptide hormones and their encoding MRNAs, as well as techniques for characterization of peptide hormone receptors. Ideal for graduate students and researchers, the book includes coverage of the general principles and strategies that the methods are based on, so the techniques will have a wide application to biologically active peptides involved in neurotransmission, growth regulations, differentiation, and vasoregulation.
This volume investigates the methodological challenges and technical requirements for the isolation, identification, and analysis of polypeptide hormones in biological research. Authors J. C. Hutton and K. Siddle provide a comprehensive framework for researchers and graduate students, detailing the strategies necessary for studying hormone secretion and receptor characterization. By synthesizing established laboratory techniques, the text serves as a foundational resource for understanding the regulatory roles of peptides in vital physiological processes.
What You Will Find
Experts and researchers in the field of endocrinology identify this text as a foundational manual for laboratory practice. Readers frequently note the technical density of the prose, which is specifically tailored for advanced students and professional scientists requiring precise experimental guidance.
Page Count:
368
Publication Date:
1990-12-27
Publisher:
Oxford University Press
ISBN-10:
0199630682
ISBN-13:
9780199630684
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