
Caenorhabditis Elegans Has Been A Popular Model Organism For Biological Research For Over Thirty Years And Has Been Used To Investigate Many Aspects Of Animal Development, For Example Apoptosis, The Hox Genes, Signal Transduction Pathways, And The Development Of The Nervous System. It Has Recently Taken On New Importance With The Publication Of The Entire Genome Sequence In 1998. The First Chapter Gives All The Basic Information On C. Elegans Required To Use It: It's Natural History, Anatomy, Life Cycle, Development, And Evolution. Information On How To Obtain, Grow, And Maintain C. Elegans For Use As A Model System Is Given In Chapter 4. Chapters 2 And 3 Describe The Genome Project And Show How To Use Genome Sequence Information By Searching The Database For Homologues Using Different Search Methods And Then How To Analyse The Search Data. The Next Chapter Gives The Essential Practical Details Of Transformation And Common Uses For The Technique. Chapter 6 Covers Reverse Genetics And Describes Strategies For Gene Inactivation That Are Known To Work In C Elegans: Epigenetic Inactivation And Mutational Germ Line Inactivation. Chapter 7 Is Designed To Help The User Analyse Phenotype By Microscopy And Includes Normaski, Fluorescence, 4-dimensional, And Electron Microscopy. Techniques For Studying The Neurobiology Of C. Elegans Are Given In Chapter 8. Chapter 9 Describes The Three Commonly Used Approaches For Studying Gene Expression And Chapter 10 Deals With The Common Methods Of Molecular Biology Essential For Gene Characterization. C. Elegans Is Not The Ideal Organism For Biochemical Studies, But Chapter 11 Describes Several Procedures For Producing Biochemically Useful Quantities Of Pure Tissues. The Final Chapter Is About Conventional Genetics And Details The Standard Procedures For Selfing And Crossing; Mutagenesis And Mutant Screening; Characterization Of Mutants; Gene Mapping; Temperature-shift Experiments And Mosaic Analysis.
This text serves as a comprehensive methodological guide for researchers utilizing the nematode Caenorhabditis elegans as a model organism in biological studies. Ian A. Hope provides a structured framework for integrating genomic data with practical laboratory techniques. The book synthesizes established biological knowledge with specific protocols for gene manipulation, microscopy, and genetic analysis to assist scientists in navigating the complexities of this model system.
What You Will Find
Scope Limits
Researchers frequently cite this work as a foundational laboratory manual for those beginning work with C. elegans. Experts highlight the text for its clear organization of complex molecular biology techniques and its utility as a bridge between genomic data and bench-side practice.
Page Count:
306
Publication Date:
1999-01-01
Publisher:
Oup Oxford
ISBN-10:
019159198X
ISBN-13:
9780191591983
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